ChloroquineV1, France, Analysis, bibRecord, 000388

Synthesis of Polyallylamine Derivatives and Their Use as Gene Transfer Vectors in Vitro

Identifieur interne : 000388 ( France/Analysis ); précédent : 000387; suivant : 000389

Synthesis of Polyallylamine Derivatives and Their Use as Gene Transfer Vectors in Vitro

Auteurs : Otmane Boussif [France] ; Thierry Delair [France] ; Catherine Brua [France] ; Laurent Veron [France] ; Andrea Pavirani [France] ; Hanno V. J. Kolbe [France]

Source :

Bioconjugate Chemistry [ 1043-1802 ] ; 1999.

RBID : ISTEX:2B6FD1C043DF7D9D0AC4C949C702BEF8D7174D51

Abstract

Cationic polymers possessing primary amine groups are inefficient in transferring nucleic acids into eukaryotic cells. With appropriate chemical modification, namely glycolylation of the amine groups of polylysine and polyallylamine, the actual number of free amino groups was decreased, hydrophilic residues were introduced, and the cytotoxicity of both polymers decreased significantly. Furthermore, in the case of polyallylamine, its ability to mediate gene transfer into cells increased by several orders of magnitude. Transfection efficiency was found to be dependent on the substitution level of amino groups and reached highest levels in the presence of lysosomotropic and/or fusogenic agents. At optimal conditions, glycolylated PAM was shown to be as efficient as the linear polyethylenimine of 22 kDa.

Url:

https://api.istex.fr/ark:/67375/TPS-DKXHL3X7-H/fulltext.pdf

DOI: 10.1021/bc9900439

Affiliations:

Links toward previous steps (curation, corpus...)

to stream Istex, to step Corpus: 000742
to stream Istex, to step Curation: 000742
to stream Istex, to step Checkpoint: 001407
to stream Main, to step Merge: 002629
to stream Main, to step Curation: 002593
to stream Main, to step Exploration: 002593
to stream France, to step Extraction: 000388

Links to Exploration step

ISTEX:2B6FD1C043DF7D9D0AC4C949C702BEF8D7174D51

Le document en format XML

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<front><div type="abstract">Cationic polymers possessing primary amine groups are inefficient in transferring nucleic acids into eukaryotic cells. With appropriate chemical modification, namely glycolylation of the amine groups of polylysine and polyallylamine, the actual number of free amino groups was decreased, hydrophilic residues were introduced, and the cytotoxicity of both polymers decreased significantly. Furthermore, in the case of polyallylamine, its ability to mediate gene transfer into cells increased by several orders of magnitude. Transfection efficiency was found to be dependent on the substitution level of amino groups and reached highest levels in the presence of lysosomotropic and/or fusogenic agents. At optimal conditions, glycolylated PAM was shown to be as efficient as the linear polyethylenimine of 22 kDa.</div>
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Synthesis of Polyallylamine Derivatives and Their Use as Gene Transfer Vectors in Vitro

Synthesis of Polyallylamine Derivatives and Their Use as Gene Transfer Vectors in Vitro

Source :

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri